190 nm to 700 nm (190 nm to 900 nm for the SPD-20AV). Spectral Bandwidth: 8 nm. Wavelength Accuracy: ±1 nm. Wavelength Reproducibility: ±0.1 nm. Noise Level: AU (under specified standard conditions). Drift: AU/hour (under specified standard conditions). 2. Installation and Initial Setup
While the SPD-20A is an exceptionally sensitive UV-VIS detector, users often consider the for peak purity verification. The SPD-M20A provides 3D data and spectral information, whereas the SPD-20A focuses on high-sensitivity quantification at specific wavelengths. 5. Summary of Specifications (According to Official Data) Light Source Deuterium ( D2cap D sub 2 D2cap D sub 2 + Tungsten ( Wavelength Range 190 to 700 nm 190 to 900 nm Noise Level Linearity 2.5 AU (ASTM) 2.5 AU (ASTM) Cell Temp Control 5∘C5 raised to the composed with power C 50∘C50 raised to the composed with power C 5∘C5 raised to the composed with power C 50∘C50 raised to the composed with power C Data compiled from Scinteck Instruments and IET Ltd .
Furthermore, the is a parameter often overlooked by novice users but emphasized in the manual. This setting acts as an electronic filter to smooth out high-frequency noise. However, there is a trade-off: a longer response time reduces noise but can blur sharp, narrow peaks, reducing resolution. Conversely, a shorter response time preserves peak shape but increases baseline noise. The manual recommends a response time set to approximately 10% of the target peak’s width at half height.
Allow the lamp to warm up (typically 30–60 minutes) and the baseline to stabilize before starting an analysis.
Avoid placing the instrument directly under air conditioning vents or in direct sunlight, as temperature fluctuations cause baseline drift. Fluidic and Electrical Connections shimadzu spd20a user manual
Shimadzu SPD-20A Service Manual | PDF | Optical Filter | Light
Press FUNC until the display reads λ1 . Input your primary wavelength.
Understanding the core specifications of the SPD-20A helps optimize chromatographic methods and ensure data integrity. Deuterium ( D2cap D sub 2 ) lamp for UV range; Tungsten ( ) lamp for visible range (on the SPD-20AV model). Wavelength Range: 190 nm to 700 nm. Spectral Bandwidth: 8 nm. Wavelength Accuracy: ±1plus or minus 1 Noise Level: AU (under specified conditions). Drift: Principle of Operation
Search "Shimadzu SPD-20A lamp replacement" on YouTube’s Shimadzu Scientific Instruments channel. These videos align exactly with the manual’s written steps but include visual guidance. 190 nm to 700 nm (190 nm to 900 nm for the SPD-20AV)
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Proper physical placement and fluidic connections prevent baseline noise and leaks. Bench Placement Place the unit on a flat, vibration-free laboratory bench.
Proper physical setup and environmental conditions prevent baseline drift and premature lamp failure. Environmental Requirements
Utilize the built-in mercury lamp calibration feature to ensure the 190-700 nm range is accurate. B. Dual-Wavelength Measurement Wavelength Reproducibility: ±0
Tares the current absorbance output to 0.0000 AU. Use this after the baseline has stabilized with the mobile phase flowing. [LAMP]: Turns the Deuterium ( D2cap D sub 2 ) or Tungsten ( ) lamps ON or OFF manually. [ENTER]: Confirms value entries and menu selections. 4. Standard Operating Procedure (SOP) Startup Sequence Turn on the main power switch of the SPD-20A. Press the [LAMP] key to ignite the D2cap D sub 2
Download the PDF today, print the troubleshooting matrix, and tape it to the side of your HPLC system. Your future self—in the middle of a midnight run with a mysterious baseline drift—will thank you.
If your baseline is drifting, it’s often dirty cell lenses rather than the lamp itself. You can dismantle the flow cell to clean or replace these lenses.