The Narishige PC-10 manual provides detailed technical specifications, including:
Set the first-stage heater value. Adjust the stop micrometer so the lower slider drops only 1 to 2 millimeters during the first burn. This thins the glass without breaking it.
Adjust the to your target value (e.g., 65.0 for standard borosilicate glass).
Minimizes output voltage variations to ensure the heater reaches the exact same intensity every time.
Understanding the physical layout is key to navigating the manual 1.2.1. narishige pc10 manual new
Insert a clean glass capillary tube through the upper slider, down through the center of the heater coil, and into the lower slider.
The original manuals? They’ve become ghosts.
Pulls the capillary in two separate heat-and-pull cycles. This is ideal for creating firm, parallel microelectrodes for patch-clamping or long, thin needles for microinjection. Precision Controls and Settings
Here are the key technical specifications for the Narishige PC-10, compiled from various sources: Adjust the to your target value (e
Gravity-operated mechanism that provides the force for the pull. 3. Initial Setup and Calibration
, the PC-10 remains a robust tool for creating tips ranging from sub-micron sizes to tens of microns. Core Functionality and Modes
The Narishige PC-10 is an indispensable tool, and familiarity with the official manual is the first step toward producing high-quality electrodes. By optimizing heater levels and mastering the two-stage process, you can achieve the specific pipette geometry needed for your experiments.
The capillary is pulled in one continuous motion. This typically produces longer, more flexible needles suitable for certain microinjection tasks. Insert a clean glass capillary tube through the
Regular maintenance ensures long-term consistency and extends the lifespan of the heating components.
The original PC-10 manual was written for 1980s lab conditions—stable mains voltage, mercury thermometers, and patience. The new community manuals assume you have a multimeter, a smartphone camera for macro shots, and maybe a 3D printer for replacement knobs.
Complete User Manual and Operating Guide for the Narishige PC-10 Glass Microelectrode Puller
Because the unit relies on radiant heat, external factors like air currents and humidity can affect performance. The PC-10 includes an acrylic shroud to shield the pipette from drafts.
For a standard (commonly used for patch pipettes with a 1–2 Set the toggle switch to 2-Stage .